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Antibodies sure to the blocking peptide now not bind to your epitope over the concentrate on protein. By comparing the staining from the blocked antibody compared to the antibody on your own, you can see which staining is distinct.
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This tutorial outlines further more doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
This guide outlines further more achievable optimization of mobile and immunoassay parameters to be sure the very best benefits are...
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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Blocking peptides are peptides that bind particularly on the goal antibody and block antibody binding. These peptide generally incorporates the epitope identified from the antibody.
Target Information and facts Cyclin E belongs into the highly conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins exhibit distinctive expression and degradation designs which lead for the temporal coordination of each mitotic occasion. Cyclin E types a posh with and capabilities for a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and is also degraded as cells progress as a result of S stage.
Overexpression of this gene has long been noticed in lots of tumors, which ends up in chromosome instability, and thus may well add to tumorigenesis. This protein was found to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a critical purpose in promoting mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already explained. Two extra splice variants were documented but comprehensive nucleotide sequence information and facts just isn't still obtainable.
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